Java is a powerful object oriented programming language that dominates many other programming languages for more than a decade. It is well designed and available as many executable technologies for software development such as Java Swing, Java Beans, Java Applets, Java Web Start, Java Database Connectivity (JDBC), Java Server Pages (JSP) and Java 2 Enterprise Edition (J2EE). Beyond its usage in the IT sector, the language is prominent even in the new emerging fields including bioinformatics and computational biology. The biological data (genes and proteins) from the biological and medical research is immense and require software professionals to mine them for new knowledge discovery. The knowledge to merge the programming concepts of Java to understand a wide range of biological concepts opens a new career challenge for many IT professionals. This paper introduces the implementation of the coding knowledge of Java in the field of molecular biology.